If standards of a known size are run previously, then a calibration curve can be created to determine the sizes of polymer molecules of interest in the solvent chosen for analysis often tetrahydrofuran (THF). Alternatively, techniques such as light scattering and/or viscometry, which do not rely on the calibration using standards of known MW, can be used online with SEC to yield absolute MWs. Because of the difference in size of two different polymers
with identical MWs, the absolute determination methods are, in general, more desirable.
If standards of a known size are run previously, then a calibration curve can be created to determine the sizes of polymer molecules of interest in the solvent chosen for analysis often tetrahydrofuran (THF). Alternatively, techniques such as light scattering and/or viscometry, which do not rely on the calibration using standards of known MW, can be used online with SEC to yield absolute MWs. Because of the difference in size of two different polymerswith identical MWs, the absolute determination methods are, in general, more desirable.
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