concentrations in 50% apple juice from 6.3 log cfu/ml to 6.9 log cfu/
ml according to the different alicyclobacillus strains, and in apple
juice from 4.7 log cfu/ml to 5.4 log cfu/ml for all three strains. Thus,
significant growth inhibition was obtained at the MICs of both the
V20 and V40 extracts, with the cell concentrations at around
3 log cfu/ml after 24 h in 50% apple juice and apple juice (p < 0.05).
Interestingly, with no additions made to the incubations, when
all three of these alicyclobacilli strains were monitored in 50% apple
juice and apple juice, the data showed lower growth kinetics when
comparing to BAT broth, as seen from the differences in the cell
concentrations for A. acidoterrestris after 24 h: 6.9 log cfu/ml in 50%
apple juice, and 5.4 log cfu/ml in apple juice, in comparison to
7.3 log cfu/ml in BAT broth (Figs. 1A and 2A). The data for the
growth kinetics of the other two strains, A. hesperidum and
A. cycloheptanicus (Figs. 1B,C and 2B,C) confirm these differences in
the vegetative cell numbers for the 50% apple juice and apple juice
compared to BAT broth, of 1.5 log cfu/ml to 2.1 log cfu/ml.