as a measure of antioxidant
power. An antioxidant reduces the ferric ion (Fe+3) to the ferrous
ion (Fe+2); the latter forms a blue complex (Fe+2/TPTZ), which increases
the absorbance at 593 nm. The supernatant (0.30 ml) and
FRAP reagent (3 ml) were added to each well and mixed thoroughly.
Absorbance was taken at 593 nm after 10 min. A standard
curve was prepared using different concentrations of Trolox. All
determinations were performed in triplicate.