Recirculation rate
was adjusted to 1200% of each tank volume per day. In addition, 5-10%
fresh seawater was supplied to recirculation system to avoid high
nitrate concentrations. Fluorescent bulbs 80 W were hung 0.5 m above
each tank to obtain the desired photoperiod 14 h light and10 h dark.
Molting, maturation and spawning of each individual female were
monitored and recorded daily. For this purpose, females were marked
by number tagging around the eyestalk.