2.4.3.2. ABTS+ radical scavenging spectrophotometric assay. The
ABTS+ radical scavenging activity of the sample was measured
according to the method developed by Re, Pellegrini, Proteggente,
Pannala, and Yang (1999). The ABTS+ solution was diluted with
redistilled water until the absorbance of 0.700 (0.02) at 743 nm
was achieved. Upon addition of 60 lk of the supernatant to 3 ml
of diluted ABTS+ solution, absorbance was read exactly 6 min after
initial mixing in the spectrophotometer (Shimadzu UV-2401 PC).
All determinations were performed in triplicate.