the pH change with time, titration of the formed acid, following
the de-emulsification of the substrate and other
techniques like HPLC or GC. Techniques like HPLC or GC are
time consuming, expensive and ineffective especially when
solving such simple questions like the quantification of the
hydrolysis of esters. pH-stat methods are simple but usually
require large sample volumes. To speed up this screening
and for a rough selection of desired enzymes, Kazlauskas and
co-workers have developed a quantitative, colorimetric
assay for hydrolysis of esters using pH indicators and photometric
detection. However, with existing spectrophotometric
methods only limited pH-ranges can be investigated, one
has to be very careful when choosing reaction conditions
the pH change with time, titration of the formed acid, following
the de-emulsification of the substrate and other
techniques like HPLC or GC. Techniques like HPLC or GC are
time consuming, expensive and ineffective especially when
solving such simple questions like the quantification of the
hydrolysis of esters. pH-stat methods are simple but usually
require large sample volumes. To speed up this screening
and for a rough selection of desired enzymes, Kazlauskas and
co-workers have developed a quantitative, colorimetric
assay for hydrolysis of esters using pH indicators and photometric
detection. However, with existing spectrophotometric
methods only limited pH-ranges can be investigated, one
has to be very careful when choosing reaction conditions
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