2.2.1. Phenolic compounds
2.2.1.1. Methanolic extraction of phenolic compounds (PCs). Phenolics
were extracted according to the procedure of Cardador Martinez et al. (2002). Raw, cooked and dry heated beans (1 g)
were placed in a 50 mL flask and mixed with 10 mL methanol.
The flask was protected from light and stirred (450 rpm, IKA-WERKE
R015, magnetic stirrer, IKAWorks, NC, USA) for 24 h at 25 C.
Mixture was centrifuged at 2166g for 10 min in a HERMLE
Z323K (Wehingen, Germany) centrifuge. Supernatant was
collected and stored at 4 C until analysis.