2.2 Preparation of ZZ
Plant material of Z. zerumbet were collected locally, authenticated
by plant taxonomist and voucher specimen was submitted to the
herbarium of the department of Botany, University of Calcutta,
Kolkata. A total of 100 gm of the dried rhizome was soaked in 1L
of ethanol at room temperature (35.5 °C) and kept in dark for 7
days. The extract was filtered and evaporated to dryness. Finally
3.45 gm of semi-solid mass of ZZ was recovered and stored at
-20 0C.