The compounds with chelating capacity may significantly
inhibit the lipid peroxidation in biological and food systems. Distinct
differences in chelating activity on Fe2+ were observed between RF
extract and LBF and WBF extracts (Table 1). Rutin as the most potent
antioxidant in buckwheat was evidenced as a strong antioxidant in
the Fenton reaction in which it could act as metal chelator and/or
radical scavenger (Yang et al., 2008). Thus, it is likely that the
enrichment of gluten-free breads with buckwheat flours in the range
of 10–30% in the mixture for the formulations contributed to the
increase of metal chelating activity on Fe2+ (Fig. 5).