Identification of Bacterial Genera
After scoring the bacterial count, the predominant
bacterial genera were identified. One bacterial colony
of each predominant genus was selected from the
agar plate and subjected to a panel of biochemical
tests including Gram’s stain, catalase, and oxidase.
These tests allow tentative classification of the bacterial
species into genus level. This was followed by further
identification using the relevant identification kit:
RapIDTM NH System was used to identify Gram-negative
cocci, RapIDTM ONE System was used to identify
Gram-negative bacilli and oxidase-negative species,
RapidTM NF Plus System was used to identify
Gram-negative and oxidase positive species and
Staphaurex (Remel, USA) was used to identify