A 125 mL aliquot of Folin-Ciocalteau 1 mol equi/L was added and,
after reacting for 3 min, 625 mL of Na2CO3 1 mol equi/L was added.
The mixture was incubated for 2 h at room temperature, and the
absorbance was read at 725 nm in a UVevisible spectrophotometer
(Amersham Biosciences, model Ultrospec 3000, England). The
quantitation of phenolic compounds was performed using a standard
curve of gallic acid with concentrations ranging from 0.02 mg/
L to 0.50 mg/L. The results were expressed as milligrams of GAE per
gram of dry sample (mg/g).