cells of all three of these strains of alicyclobacilli. Both of these
extracts showed the greatest effects during the first 8 h of incubation,
with cell concentrations of 3.2 log cfu/ml and 2.5 log cfu/ml
for V20 and V40, respectively (Fig. 1A). The antibacterial effects
remained almost the same over the final 16 h of incubation, and
reached cell concentrations of 3.4 log cfu/ml and 2.6 log cfu/ml for
V20 and V40, respectively (Fig. 1A). Similar cell concentrations
were confirmed with V20 and V40 for A. hesperidum and
A. cycloheptanicus (Fig. 1B and C). This growth inhibition showed
greater effects of V40 over V20 on these alicyclobacilli, except for
A. hesperidum. Therewas a significant difference between mean cell