Bead-beating method
Bead-beating disruption was performed between
procedures
①
and
②
in the GES method
described above and the yields of DNA were
compared. A comparison was made between glass
and zirconia as beads materials used for
disruption. Beads Cell Disrupter Micro Smash
(Tomy Seiko Co., Ltd., Tokyo) was used as a
bead-beating disruption apparatus. Glass or
zirconia beads were placed
in 2.0ml sample tubes
(Cat No. 72693 or 72694, Sarstedt, Inc.) and
bacteria suspension in buffer was added to the
tubes, which were then ti
ghtly capped and placed
symmetrically in the tube holder of the apparatus
to properly balance them. After the power knob of
the apparatus was fully tightened to secure it in
place, the lid of the disruption chamber was
closed and the samples were bead beaten at the
set speed (rpm) and time (sec).