PS. Moreover, the lowest proportion of POPs corresponded to sitostanol,
independently of the temperature and of whether this PS was free or
esterified.
We know of no other studies identifying and/or quantifying POPs
in food matrixes similar to those analyzed in the present study. As
both MFbA and MFbB contain milk, the discussion of results can be
made between the POP contents of our samples and the scant information
available in the literature involving dairy matrixes. In milks
enriched with phytosterols (free or esterified), Soupas et al. (2006)
identified β-sitosterol oxides (hydroxide, epoxy and 7-keto derivatives),
though only the 7-hydroxy and 7-ketoderivatives were quanti-
fied. The total content, which was considered low, was 218 μg POPs/
100 g of milk in the samples enriched with free phytosterols, and
203 μg POPs/100 g of milk in the samples enriched with esterified
phytosterols — such contents being lower than those recorded in
our study. The lesser enrichment in phytosterols (0.5%) versus that
used in our study (1.77–1.84%) would justify the observed differences
in POP contents. Accordingly, Menéndez-Carreño et al. (2008) detected
total POP contents of 180 μg/100 g in an enriched milk with phytosterol
esters (0.3%), which is similar to the observations of Soupas et al.
(2006). Regarding individual POP abundance, 7β-hydroxysitosterol
was the main POP, followed by 7-ketositosterol
PS. Moreover, the lowest proportion of POPs corresponded to sitostanol,
independently of the temperature and of whether this PS was free or
esterified.
We know of no other studies identifying and/or quantifying POPs
in food matrixes similar to those analyzed in the present study. As
both MFbA and MFbB contain milk, the discussion of results can be
made between the POP contents of our samples and the scant information
available in the literature involving dairy matrixes. In milks
enriched with phytosterols (free or esterified), Soupas et al. (2006)
identified β-sitosterol oxides (hydroxide, epoxy and 7-keto derivatives),
though only the 7-hydroxy and 7-ketoderivatives were quanti-
fied. The total content, which was considered low, was 218 μg POPs/
100 g of milk in the samples enriched with free phytosterols, and
203 μg POPs/100 g of milk in the samples enriched with esterified
phytosterols — such contents being lower than those recorded in
our study. The lesser enrichment in phytosterols (0.5%) versus that
used in our study (1.77–1.84%) would justify the observed differences
in POP contents. Accordingly, Menéndez-Carreño et al. (2008) detected
total POP contents of 180 μg/100 g in an enriched milk with phytosterol
esters (0.3%), which is similar to the observations of Soupas et al.
(2006). Regarding individual POP abundance, 7β-hydroxysitosterol
was the main POP, followed by 7-ketositosterol
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