The formation of niosomal vesicles as well as their
morphological aspects were evaluated by using photo and
transmission electron microscopy.
Various niosomal formulations were examined under
optical microscope (Lecia Image, Germany) and photographed
at a magnification of ×40, by means of a fitted
camera (JVC, Japan).
Gentamicin sulphate niosomal samples were also examined
by transmission electron microscope (model JEM-1230,
Jeol, Tokyo, Japan) at 70 kV, after being negatively stained.
A saturated ammonium molybdate aqueous solution was
used as the staining agent (23).