It was observed that over the study period (48 h) none of
the tested formulae showed any signs of redness, inflammation
or increased tear production. Thus it could concluded
that the non ionic surfactants namely Tween 60 [F4], Tween
80 [F6], and Brij 35 [F12], used in the niosomal formulations
as well as the other excipients were non-irritant to the eye