who found out that wheat bread enriched with wholegrainbuckwheat flour was more effective concerning antioxidant activity in
comparison to wheat bread enriched with light buckwheat flour.
Results obtained by Lin et al. (2009) showed that IC50 value of
scavenging activity on DPPH• for husked and unhusked buckwheat
enhanced wheat breads were 20.29 and 9.75 mg/ml, respectively.
According to results presented in Fig. 4 similar IC50 values of
scavenging activity on DPPH• for gluten-free bread were expressed.
In comparison to results of Lin et al. (2009) which DPPH values
originate from phenolic compounds of both buckwheat as well as
wheat flour, our results for gluten-free bread samples expressed
similar values. However as rice flour is known to possess less phenolic
compounds than wheat flour, the obtained results are mainly
influenced by the antioxidative potential of used buckwheat flours.
Considering the ratio of calculated and determined IC50 values, trend
of yield instead of loss for IC50 values was observed. This percentage of
yield of IC50 values of scavenging activity on DPPH• increased with
increase of both buckwheat flour contents and it was more expressed
for WBF containing products. This phenomenon can be explained by
previously mentioned reactions of synthesis during the heat treatment
(Gawlik-Dziki et al., 2009; Manzocco et al., 2001; Zieliński et al.,
2001). The compounds with chelating capacity may significantly
inhibit the lipid peroxidation in biological and food systems. Distinct
differences in chelating activity on Fe2+ were observed between RF
extract and LBF and WBF extracts (Table 1). Rutin as the most potent
antioxidant in buckwheat was evidenced as a strong antioxidant in
the Fenton reaction in which it could act as metal chelator and/or
radical scavenger (Yang et al., 2008).
who found out that wheat bread enriched with wholegrainbuckwheat flour was more effective concerning antioxidant activity incomparison to wheat bread enriched with light buckwheat flour.Results obtained by Lin et al. (2009) showed that IC50 value ofscavenging activity on DPPH• for husked and unhusked buckwheatenhanced wheat breads were 20.29 and 9.75 mg/ml, respectively.According to results presented in Fig. 4 similar IC50 values ofscavenging activity on DPPH• for gluten-free bread were expressed.In comparison to results of Lin et al. (2009) which DPPH valuesoriginate from phenolic compounds of both buckwheat as well aswheat flour, our results for gluten-free bread samples expressedsimilar values. However as rice flour is known to possess less phenoliccompounds than wheat flour, the obtained results are mainlyinfluenced by the antioxidative potential of used buckwheat flours.Considering the ratio of calculated and determined IC50 values, trendof yield instead of loss for IC50 values was observed. This percentage ofyield of IC50 values of scavenging activity on DPPH• increased withincrease of both buckwheat flour contents and it was more expressedfor WBF containing products. This phenomenon can be explained bypreviously mentioned reactions of synthesis during the heat treatment(Gawlik-Dziki et al., 2009; Manzocco et al., 2001; Zieliński et al.,2001). The compounds with chelating capacity may significantlyinhibit the lipid peroxidation in biological and food systems. Distinctdifferences in chelating activity on Fe2+ were observed between RFextract and LBF and WBF extracts (Table 1). Rutin as the most potentantioxidant in buckwheat was evidenced as a strong antioxidant inthe Fenton reaction in which it could act as metal chelator and/orradical scavenger (Yang et al., 2008).
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